KMID : 0368419930360040371
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Journal of Plant Biology 1993 Volume.36 No. 4 p.371 ~ p.375
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The Reason for the Loss of Photosynthetic Activity in Isolated Spinach Chloroplasts during Photosynthesis
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Jun, Sung Soo/îïàøáú
Lee, Chin Bum/Hong, Young Nam/ì°òåÛô/ûóçÈÑû
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Abstract
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CO_2 fixation of isolated intact spinach chloroplasts under saturating light began to decrease after 20 min, and stopped completely after 1 h. To identify the lesion sites for the die off, reconstituted chloroplast system was used with chloroplasts collected at several phases of time course. CO_2 fixation was inhibited in the reconstituted chloroplasts made of thylakoids and stroma in the later phases, but showed a higher degree of inhibitio by the participation of thylakoids than that of stroma in the later phases. Measurement of photophosphrylation and NADP reduction revealed that a severed thylakoidal damage was occurred at the later phases. This results indicate that the lesion sites for the die off are in the thylakoid.
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